Craig Ventors First Cell

Cr Craig Ventor first first self-replicating, synthetic bacterial cell ROCKVILLE, MD and San Diego, CA (May 20, 2010)— Researchers at the J. Craig Venter Institute (JCVI), a not-for-profit genomic research organization, published results today describing the successful construction of the first self-replicating, synthetic bacterial cell. The team synthesized the 1. 08 million base pair chromosome of a modified Mycoplasma mycoides genome. The synthetic cell is called Mycoplasma mycoides JCVI-syn1. and is the proof of principle that genomes can be designed in the computer, chemically made in the laboratory and transplanted into a recipient cell to produce a new self-replicating cell controlled only by the synthetic genome. This research will be published by Daniel Gibson et al in the May 20th edition of Science Express and will appear in an upcoming print issue of Science. â€Å"For nearly 15 years Ham Smith, Clyde Hutchison and the rest of our team have been working toward thi s publication today–the successful completion of our work to construct a bacterial cell that is fully controlled by a synthetic genome,† said J.Craig Venter, Ph. D. , founder and president, JCVI and senior author on the paper. â€Å"We have been consumed by this research, but we have also been equally focused on addressing the societal implications of what we believe will be one of the most powerful technologies and industrial drivers for societal good. We look forward to continued review and dialogue about the important applications of this work to ensure that it is used for the benefit of all. † According to Dr.Smith, â€Å"With this first synthetic bacterial cell and the new tools and technologies we developed to successfully complete this project, we now have the means to dissect the genetic instruction set of a bacterial cell to see and understand how it really works. † To complete this final stage in the nearly 15 year process to construct and boot u p a synthetic cell, JCVI scientists began with the accurate, digitized genome of the bacterium, M. mycoides. The team designed 1,078 specific cassettes of DNA that were 1,080 base pairs long. These cassettes were designed so that the ends of each DNA cassette overlapped each of its neighbors by 80bp.The cassettes were made according to JCVI’s specifications by the DNA synthesis company, Blue Heron Biotechnology. The JCVI team employed a three stage process using their previously described yeast assembly system to build the genome using the 1,078 cassettes. The first stage involved taking 10 cassettes of DNA at a time to build 110, 10,000 bp segments. In the second stage, these 10,000 bp segments are taken 10 at a time to produce eleven, 100,000 bp segments. In the final step, all 11, 100 kb segments were assembled into the complete synthetic genome in yeast cells and grown as a yeast artificial chromosome.The complete synthetic M. mycoides genome was isolated from the yeast c ell and transplanted into Mycoplasma capricolum recipient cells that have had the genes for its restriction enzyme removed. The synthetic genome DNA was transcribed into messenger RNA, which in turn was translated into new proteins. The M. capricolum genome was either destroyed by M. mycoides restriction enzymes or was lost during cell replication. After two days viable M. mycoides cells, which contained only synthetic DNA, were clearly visible on petri dishes containing bacterial growth medium.The initial synthesis of the synthetic genome did not result in any viable cells so the JCVI team developed an error correction method to test that each cassette they constructed was biologically functional. They did this by using a combination of 100 kb natural and synthetic segments of DNA to produce semi-synthetic genomes. This approach allowed for the testing of each synthetic segment in combination with 10 natural segments for their capacity to be transplanted and form new cells. Ten out of 11 synthetic fragments resulted in viable cells; therefore the team narrowed the issue down to a single 100 kb cassette.DNA sequencing revealed that a single base pair deletion in an essential gene was responsible for the unsuccessful transplants. Once this one base pair error was corrected, the first viable synthetic cell was produced. Dr. Gibson stated, â€Å"To produce a synthetic cell, our group had to learn how to sequence, synthesize, and transplant genomes. Many hurdles had to be overcome, but we are now able to combine all of these steps to produce synthetic cells in the laboratory. † He added, â€Å"We can now begin working on our ultimate objective of synthesizing a minimal cell containing only the genes necessary to sustain life in its simplest form.This will help us better understand how cells work. † This publication represents the construction of the largest synthetic molecule of a defined structure; the genome is almost double the size of the previou s Mycoplasma genitalium synthesis. With this successful proof of principle, the group will now work on creating a minimal genome, which has been a goal since 1995. They will do this by whittling away at the synthetic genome and repeating transplantation experiments until no more genes can be disrupted and the genome is as small as possible. This minimal cell will be a platform for analyzing the function of every essential gene in a cell.According to Dr. Hutchison, â€Å"To me the most remarkable thing about our synthetic cell is that its genome was designed in the computer and brought to life through chemical synthesis, without using any pieces of natural DNA. This involved developing many new and useful methods along the way. We have assembled an amazing group of scientists that have made this possible. † As in the team’s 2008 publication in which they described the successful synthesis of the M. genitalium genome, they designed and inserted into the genome what they called watermarks.These are specifically designed segments of DNA that use the â€Å"alphabet† of genes and proteins that enable the researcher to spell out words and phrases. The watermarks are an essential means to prove that the genome is synthetic and not native, and to identify the laboratory of origin. Encoded in the watermarks is a new DNA code for writing words, sentences and numbers. In addition to the new code there is a web address to send emails to if you can successfully decode the new code, the names of 46 authors and other key contributors and three quotations: â€Å"TO LIVE, TO ERR, TO FALL, TO TRIUMPH, TO RECREATE LIFE OUT OFLIFE. † – JAMES JOYCE; â€Å"SEE THINGS NOT AS THEY ARE, BUT AS THEY MIGHT BE. †-A quote from the book, â€Å"American Prometheus†; â€Å"WHAT I CANNOT BUILD, I CANNOT UNDERSTAND. † – RICHARD FEYNMAN. The JCVI scientists envision that the knowledge gained by constructing this first self-replicatin g synthetic cell, coupled with decreasing costs for DNA synthesis, will give rise to wider use of this powerful technology. This will undoubtedly lead to the development of many important applications and products including biofuels, vaccines, pharmaceuticals, clean water and food products.The group continues to drive and support ethical discussion and review to ensure a positive outcome for society. Funding for this research came from Synthetic Genomics Inc. , a company co-founded by Drs. Venter and Smith. Background The research published today was made possible by previous breakthroughs at JCVI. In 2007 the team published results from the transplantation of the native M. mycoides genome into the M. capricolum cell which resulted in the M. capricolum cell being transformed into M. mycoides. This work established the notion that DNA is the software of life and that DNA dictates the cell phenotype.In 2008 the same team reported on the construction of the first synthetic bacterial ge nome by assembling DNA fragments made from the four chemicals of life—ACGT. The final assembly of DNA fragments into the whole genome was performed in yeast by making use of the yeast genetic systems. However, when the team attempted to transplant the synthetic bacterial genome out of yeast and into a recipient bacterial cell, viable transplants could not be recovered. Ethical Considerations: Since the beginning of the quest to understand and build a synthetic genome, Dr.Venter and his team have been concerned with the societal issues surrounding the work. In 1995 while the team was doing the research on the minimal genome, the work underwent significant ethical review by a panel of experts at the University of Pennsylvania (Cho et al, Science December 1999:Vol. 286. no. 5447, pp. 2087 – 2090). The bioethical group's independent deliberations, published at the same time as the scientific minimal genome research, resulted in a unanimous decision that there were no stron g ethical reasons why the work should not continue as long as the scientists involved continued to engage public discussion. Dr.Venter and the team at JCVI continue to work with bioethicists, outside policy groups, legislative members and staff, and the public to encourage discussion and understanding about the societal implications of their work and the field of synthetic genomics generally. As such, the JCVI’s policy team, along with the Center for Strategic & International Studies (CSIS), and the Massachusetts Institute of Technology (MIT), were funded by a grant from the Alfred P. Sloan Foundation for a 20-month study that explored the risks and benefits of this emerging technology, as well as possible safeguards to prevent abuse, including bioterrorism.After several workshops and public sessions the group published a report in October 2007 outlining options for the field and its researchers. Most recently in December of 2008, JCVI received funding from the Alfred P. Sloa n Foundation to examine ethical and societal concerns that are associated with the developing science of synthetic genomics. The ongoing research is intended to inform the scientific community as well as educate our policymakers and journalists so that they may engage in informed discussions on the topic.About the J. Craig Venter Institute The JCVI is a not-for-profit research institute in Rockville, MD and La Jolla, CA dedicated to the advancement of the science of genomics; the understanding of its implications for society; and communication of those results to the scientific community, the public, and policymakers. Founded by J. Craig Venter, Ph. D. , the JCVI is home to approximately 400 scientists and staff with expertise in human and evolutionary biology, genetics, ioinformatics/informatics, information technology, high-throughput DNA sequencing, genomic and environmental policy research, and public education in science and science policy. The legacy organizations of the JCVI are: The Institute for Genomic Research (TIGR), The Center for the Advancement of Genomics (TCAG), the Institute for Biological Energy Alternatives (IBEA), the Joint Technology Center (JTC), and the J. Craig Venter Science Foundation. The JCVI is a 501 (c) (3) organization. For additional information, please visit http://www. JCVI. org. Media Contact: Heather Kowalski, 301-943-8879, hkowalski(AT)jcvi. org

Risk Management Strategy and Plan Document Shell

Project Selection: The first step will be to select a project that you to develop a risk management strategy and plan for. This project will be used as the basis for each of the assignments throughout the course and should conform to the following guidelines: 1. Nontrivial: The selected project candidate should reflect a real-life project that has at least the major milestones identified. 2. Domain Knowledge: You will be applying knowledge of this project to address assignments related to identifying and analyzing risks.Select a project that fits these requirements, and submit your proposal to your instructor before proceeding further with the assignments in the course. Approval should be sought within the first few days of the course. Your instructor will tell you how to submit this proposal and what notification will be given for project approval. Assignment: Your first task in this process will be to select a project to use as the basis of your Key Assignment.You will also create the shell document for the final project Key Assignment document deliverable that you will be working on throughout the course. As you proceed through each project phase, you will add content to each section of the final document to gradually complete the final project delivery, which is the Risk Management Strategy and Plan. The following project deliverables: 1. Submit project proposal to instructor for approval 2. Risk Management Strategy and Plan Document Shell * Use Microsoft Word * Title Page 1.Course number and name 2. Project name 3. Student name 4. Date * Table of Contents 1. Use auto-generated TOC 2. Separate page 3. Maximum of three levels deep 4. Be sure to update the fields of the TOC so it is up-to-date before submitting your project. 1. Section Headings (create each heading on a new page with TBD as content except for sections listed under â€Å"New Content† below) 1. Project Outline 2. Risk Management Justification 3. Project Risks Identification 4. Project Ri sks Assessment 5. Project Risks Responses Strategy . Project Risks Management Plan 7. Project Risks Monitoring & Control Plan 8. Project Risks WBS & Budget Updates 9. Project Risks Communications Plan 1. New Content to be Inserted in Above Shell * Project Outline 1. Brief description of the project. 2. The milestones and/or WBS for the project. 3. Material can be taken from approved proposal submitted to the instructor, and this will serve as the draft for the proposal. 4. Be sure this project is approved by the instructor. 1. Risk Management Justification 1.Discuss why risk management is so important to the success of the selected project in a letter to the project sponsor. 2. Describe the steps that will be used to develop the Risk Management Plan. 3. Represent this process in a flow diagram as well 1. Project Risks Identification * Start brainstorming, at a high level, on some categories where risk could occur in this project (Note: you will continue this brainstorming activity i n the next week with specifics, likelihood, and impact). Name the document yourname_MPM344_IP1. doc, and submit the document for grading.

Ross Douthat Rhetorical Analysis - 1335 Words

Even though Ross Douthat covers a wide range of topics he tends to start out with his main point or theme. He will argue his point until he feels that he has convinced his audience of at least understanding his point of view even if it doesn’t change their own opinion. He has showed that he wants his audience to agree with him on every topic that I have read since he argues his point and puts his opinion into his writing. While he’s trying to get his audience to agree with him, he makes it easy to follow what he’s saying, because he wants you to understand what he is saying and what he means when he says it so that you will be more likely to agree with his opinion. When Douthat is arguing his point it’s normally organized and rarely jumps†¦show more content†¦Douthat tends to speak a lot as an individual rather than a group, because he’s very opinionated he tries to let his opinion be known and he wants them to know that his opinion is also backed with some facts he tries really hard to always have some proof on why his opinion is true, but sometimes it is just an opinion that can’t be backed by any proof (Like a political stance). When Douthat writes he is good at keeping his emotion out of what he is writing, unless it gets personal or if he strongly doesn’t like something about it. There have been a few times when he put his personal opinion into the paper and then continued talking about it before he stated another fact or source. When Douthat starts talking about politics, especially about Trump, he starts to describe them with more opinionated ways than factual ways, for example, in his article, â€Å"Trump’s Empty Culture Wars†, (09/27/17), he says, â€Å"Unfortunately for us all Donald Trump is a master, a virtuoso, of the second kind of culture war - and a master, too, of taking social and cultural debates that could be important and necessary and making them stupider and emptier and all about himself† Which shows his opinion very heavily

Bilingual Rap Poetry, Pragmatics And Performativity

Natalie Skouson LING 452 Final Article 1: Multilingual Codeswitching in Quebec Rap: Poetry, Pragmatics and Performativity This article was chosen because of the four it seemed to be the most unique. It does not look like it would discuss any subject that I have read academic articles on before. This would mean that I would have the opportunity to learn new information and that the thoughts and insights that I have from this article are unique to it and not other things that that I have read over the years. This is a fairly hard task to accomplish because I do read a lot and there are not very many subjects that I have not read something on. This article started with discussing the reason that this research subject was chosen. The reason†¦show more content†¦This should give more background for the purpose of the research that they are doing. They then go into a short discussion of the research questions that they are focusing on. These questions are, â€Å"(1) What is the nature and function of codeswitc hing, or ‘artistic codemixing’, in Quebec Hip-Hop? (2) how does codeswitching in Hip-Hop contribute to the creation of a new community among young Quebecois? and (3) within the Quebec Hip-Hop community, what does language use tell us about identity construction?† The authors then continue to talk about codeswitching and the results of their research, moving into a discussion of the results that they found and the application of those results. The findings presented in the article did not surprise me because much of what the textbook had to say about code-switching fell along the same lines. The authors mentioned that the rap artists used this form of code-switching to add identity to their rap. The code-switching that they used is unique to them and their style. In the textbook it says: â€Å"But code-switching is often motivated by more volatile components, such as topic, or the speaker’s attitude toward the topic, or identity creation, or solidarity [emp hasis added]†¦Ã¢â‚¬  (Van Herk 2012). I think this last point by Van Herk is important to recognize. While the code-switching in rap may have initially been used to create a unique identity for the artist, as more and more artists start to follow in the footsteps

The Secret to Expository Essay Samples Geography

The Secret to Expository Essay Samples Geography Introducing Expository Essay Samples Geography Explain why some students are made to leave school as soon as they are sixteen. Your essay is supposed to concentrate on why schools insist their students learn a foreign language and the way it can really help in someone's overall improvement. Explain the reason it's essential for students to understand how to do math. Most students have a hard time writing papers on geography because of its complexity. Essay writing is a difficult business whenever you're at college. More than every other sort of writing, expository writing is a daily requirement of the majority of careers. Essay writing provides a great deal of benefits to students in the academe. If you would like your expository essay to receive high grades and grab interest of the audience, you need to always opt for the topic you want. When you proceed through various samples, you won't only get ideas on distinct topics but also know how to apply unique practices to boost your writing skills. The majority of the moment, expository essays are presented by offering a wide range of topics and methods to bring up the idea. You must always start your work with collecting suitable material. As a consequence, their papers are unstructured and frequently pointless. What to Expect From Expository Essay Samples Geography? If you browse for the forms of paragraphs' you will be surprised how many results you will receive. Each paragraph needs to be restricted to the exposition of one general idea. The conclusion is a last paragraph in which you don't will need to offer new details. It should be a construction made of the past few paragraphs. The Good, the Bad and Expository Essay Samples Geography The primary goal of such an endeavor is to supply analysis and data on a particular topic. Also, it's very useful to create a graphic organizer for help. Planning the introduction, you ought to pay exclusive attention to your primary statement. Our expository service is among the most highly reputed on the planet. In this kind of essay, you should describe something without being metaphoric. In an expository essay, you're attempting to spell out how something works or why you think that it's a great idea. In school, it's an unavoidable truth that you're going to be requested to write something about a topic which sometimes you're so eager to finish. Preparing cake all on your own will end up being t he simplest and sweetest gift to reveal your love and attention for your nearest and dearest on their birthday and they will flood you with admiration and love. What About Expository Essay Samples Geography? There are several methods about how to compose an expository essay. There are plenty of ideas for expository essay writing and hence an assortment of topics. As soon as you've chosen one subject from the scope of expository essay topics, it's time to get started writing. Consequently, students may produce different thesis for unique themes to compose essays on hamlet. Expository essay topics could be selected by means of a student as inquiry. While there are lots of diverse varieties of essays, an expository essay is perhaps among the most systematic. There are a couple distinct procedures for writing an expository essay. It is the same as writing any typical 5-paragrah essay. An essay must be clear and unbiased. Your essay will have each of the vital capabilities. Expository essays offer more thorough understanding of a specific topic. While an expository essay ought to be clear and concise, it may also be lively and engaging. For you to produce a great expository essay you've got to think about the topic as it is related to the points offered and the more the points, the simpler the writing. As you have shaped understanding of this issue and made a sort of draft, you can begin writing. You should do your best, so make certain your thoughts are clear, and logical. Before you commence writing, it is recommended to look at the expectat ions and needs of the readers. Expository writing is also the most common kind of academic writing! Writing your essay by yourself isn't always possible due to many constraints like time crunch, an emergency, etc.. Writing the body gets a little more challenging. Typically, you'll want to pick 1 method for each bit of expository writing. You're anticipated to persuade the readers with your arguments, so be certain to include as many supporting academic parts of evidence as you're able to. Try to remember, though you might not be crafting the upcoming great novel, you making an effort to leave an enduring impression on the folks evaluating your essay. If you build a productive outline for your research, you should remember the simple fact that you'll never get lost. The key thing is to remain focused on your topic and adhere to the facts. You will need to present the advantages and disadvantages of such a move, then draw a conclusion accordingly. Thus, you should comprehend each word in your topic. Make certain you understand your topic.

The Theme of Society in Pride and Prejudice free essay sample

Originally written in the late 1700s, Jane Austen’s Pride and Prejudice satirically depicts the universal ideals in Old Regency England, primarily regarding social class. Austen follows the development of an outspoken middle-class British woman, Elizabeth Bennet, as she encounters and overcomes the many social barriers that separate her from her wealthy upper-class neighbors. Throughout the novel, Lizzie must confront society’s class-consciousness, particularly with her family’s growing relationship with the wellborn Bingleys and their friend, Mr.Darcy. It is clear that the author, Jane Austen, intended Pride and Prejudice to be a parody of the Old English society’s extreme emphasis on the social class structure and marriage that is not based on the heart but instead on convenience. Although our present-day social class system is more flexible than it was back then, members of the elite, especially celebrities, are still more apt to marry other upper-class citizens, rather than their social inferiors. For example, in today’s society it is the standard for rock stars, actors and models to pursue partners from a comparable social class. Similarly, a marriage between Fitzwilliam Darcy and Anne de Bourgh, daughter of the distinguished Lady Catherine de Bourgh, is expected because both parties are of equally notable lineage and hail from the same prestigious family. The union between the two aristocrats was planned â€Å"while in their cradles†, according to Lady Catherine de Bourgh, who makes a trip to Longbourn to see Elizabeth after hearing that she is engaged to Anne’s â€Å"future husband†.Lady Catherine is horrified that the anticipated matrimony may â€Å"be prevented by a young woman of inferior birth, of no importance in the world, and wholly unallied to the family† and makes every effort to prevent any chance of an engagement between Elizabeth and Darcy. During this confrontation, Lady de Bourgh’s behavior towards Elizabeth is quite a stonishing and completely supports society’s upper class prejudices and narrow-minded ignorant views towards the lower classes. If Lady de Bourgh had not had such stately ancestry, she may have lowered her social status with her ridiculous conduct.Lady Catherine’s ludicrousness is most likely taken from her haughty ego, which society has helped create by holding the upper class up on a pedestal. In Pride and Prejudice, Jane Austen speaks of a world where a person’s, more importantly a woman’s, reputation is a paramount obsession. A woman is made to believe to have to behave in certain ways; stepping outside the social normality makes her open to being banished from mainstream society. A prime example of this is when Lydia gets an invitation to visit and stay with the officers. Of course, Mrs.Bennet, the most obvious, oblivious, loud social conscious creature of Longbourn is all â€Å"happy go lucky† when she hears of Lydia’s call. Mr. Bennet who is the most stoic contradictory of Mrs. Bennet is even excited. However, when the news provoking invitation turns into a merciless elopement between Mr. Wickham and Lydia Bennet it sends shockwaves through the Bennet household. By becoming Wickham’s lover without being married, Lydia clearly places herself outside the social â€Å"norm†, and her disgrace threatens the entire Bennet family and their standing in society.The fact that Lydia’s terrible judgment would have condemned the other Bennet sisters to marriage less lives is a ridiculous but accurate tell of the stress put on the hierarchy that is Old English society. This theme also appears in the novel when Elizabeth walks to Netherfield and arrives with a muddy skirt, to the horror of the reputation-conscious Miss Bingley. At other points, the ill-mannered, ridiculous behavior of Mrs. Bennet gives her a bad reputation with the snobbish Darcys and Bingleys.Austen pokes gentle fun at the snobs in these examples, but when Lydia elopes with Wickham and lives with him out of wedlock, the author treats reputation and class as a very serious matter. Society is closely related to reputation, in that both have the strictly scrutinized way of life for the social classes of England. Even though the gap between the upper classmen Bingleys and the lower or middle classmen Bennets is as wide as the Amazon River, the Bennets may socialize with the Bingleys.However, they are obviously the lower level socialites and are treated as so. A prominent example of this discrimination was the Bingley sisters’ rude and disrespectful manner towards Elizabeth while she stayed at Pemberley to comfort Jane when she was overcoming her sickness. Austen again satirizes this kind of severe class-consciousness in the character of Mr. Collins, who spends most of his time sucking up to his upper-class patron Lady Catherine de Bourgh. Though Mr.Collins offers an extreme example, he is not the only one to hold such views. His view of the importance of class is shared by Mr. Darcy, who believes in the dignity of his roots; Miss Bingley, who dislikes anyone not as socially accepted as she is; and Wickham, who will do anything he can to get enough money to raise himself into a higher station. The satire directed at Mr. Collins is therefore also more subtly directed at the entire social hierarchy and the conception of all those within it, in complete disregard of other, more worthy virtues.Through the Darcy-Elizabeth and Bingley-Jane marriages, Austen shows the power of love and happiness can actually overcome class boundaries and prejudices, thereby showing that even if prejudices are hollow, unfeeling, and unproductive they can turn out to mean nothing to some people. Nonetheless, people similar to Miss Bingley as well as her friends are still trapped in the warped obsessive ladder that is the autocratic society that Jane Austen portrays throughout Pride and Prejudice. Words: 941

Their Eyes Were Watching God Quotes

'Their Eyes Were Watching God' Quotes Their Eyes Were Watching God is a novel by Zora Neale Hurston. Its the story of Janie Crawford, who returns home and tells the story of the last 20 years. Quotes From the Novel These sitters had been tongueless, earless, eyeless conveniences all day long. Mules and other brutes had occupied their skins. But now, the sun and the bossman were gone, so the skins felt powerful and human. They became lords of sounds and lesser things. They passed nations through their mouths. They sat in judgment.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 1Janie saw her life like a great tree in leaf with the things suffered, things enjoyed, things done and undone. Dawn and doom was in the branches.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 2You know, honey, us colored folks is branches without roots and that makes things come round in queer ways. You in particular. Ah was born back due in slavery so it wasnt for me to fulfill my dreams of whut a woman oughta be and to do.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 2Ah wanted to preach a great sermon about colored women sittin on high, but they wasnt no pulpit for me.- Zora Neale Hurston, Th eir Eyes Were Watching God, Ch. 2 Ah wants things sweet wid mah marriage lak when you sit under a pear tree and think. Ah.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 3Janie pulled back a long time because he did not represent sun-up and pollen and blooming trees, but he spoke for far horizon. He spoke for change and chance.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 4Thank yuh fuh yo compliments, but mah wife dont know nothin bout no speech- makin.. Ah never married her for nothin lak dat. Shes uh woman and her place is in de home.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 5Take for instance that new house of his. It had two stories with porches, with bannisters and such things. The rest of the town looked like servants quarters surrounding the big house. And different from everybody else in the town he put off moving in until it had been painted, in and out. And look at the way he painted it- a gloaty, sparkly white.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 5 Janie loved the conversation and sometimes she thought up good stories on the mule, but Joe had forbidden her to indulge. He didnt want her talking after such trashy people.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 6Somebody got to think for women and chillun and chickens and cows. I god, they sho dont think none theirselves.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 6Janie did what she had never done before, that is, thrust herself into the conversation.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 6When you pull down yo britches, you look lak de change uh life.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 7It was so crazy digging worms by lamp light and setting out for Lake Sabelia after midnight that she felt like a child breaking rules. Thats what made Janie like it.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 11He could be a bee to a blossom- a pear tree blossom in the spring. He seemed to be crushing scent out of the wor ld with his footsteps. Crushing aromatic herbs with every step he took. Spices hung about him. He was a glance from God.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 11 Before the week was over he had whipped Janie. Not because her behavior justified his jealousy, but it relieved that awful fear inside him. Being able to whip her reassured him in possession. No brutal beating at all. He just slapped her around a bit to show he was boss.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 17They huddled closer and stared at the door. They just didnt use another part of their bodies, and they didnt look at anything but the door. The time was past for asking the white folks what to look for through that door. Six eyes were questioning God.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 18Once upon uh time, Ah never spected nothin, Tea Cake, but bein dead from standin still and tryin tuh laugh. But you come long and made somethin outa me. So Ahm thankful fuh anything we come through together.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 18Janie held his head tightly to her breast and wept and thanked him wordlessly for giving her th e chance for loving service. She had to hug him tight for soon he would be gone, and she had to tell him for the last time. Then the grief of outer darkness descended.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 19 So Ahm back home agin and Ahm satisfied tuh be heah. Ah done been tuh de horizon and back and now Ah kin set heah in mah house and live by comparisons.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 20Here was peace. She pulled in her horizon like a great fish-net. Pulled it from around the waist of the world and draped it over her shoulder. So much of life in its meshes! She called in her soul to come and see.- Zora Neale Hurston, Their Eyes Were Watching God, Ch. 20